5/15/2023 0 Comments Srdx motifFurthermore, the transgenic plants that expressed TTG1, which is a WD40 protein and interacts with bHLH transcription factors, fused to SRDX exhibited a phenotype similar to ttg1 mutants by transrepression and the regions of TTG1 required for interaction to the bHLH protein were detected using our system. We demonstrated the transrepressive activity of SRDX using FOS and JUN as a model system and used two MADS box plant proteins, PISTILLATA and APETALA3, which are known to form heterodimers. When we fused a protein that interacts with a transcription factor with SRDX and co-expressed the product with the transcription factor in plant cells, the expression of genes that are targets of the transcription factor was suppressed by transrepression. We demonstrate here that the ERF- associated amphiphilic repression (EAR) motif repression domain (SRDX) can convert a transcriptional complex into a repressor via transrepression that is mediated by protein–protein interactions and show that transrepressive activity of SRDX can be used to detect such protein–protein interactions. These results suggested a possible role for Cattleya orchid CaFYF1/2 in controlling floral senescence/abscission by suppressing ethylene signaling and abscission-associated genes as well as controlling flower organ elongation through negative regulation of GA response by activating the expression of the DELLA genes during flower development.The activities of many regulatory factors involve interactions with other proteins. Furthermore, 35S::CaFYF1/2 and 35S::CaFYF1/2+SRDX transgenic Arabidopsis plants showed additional morphological defects, such as short sepals and petals, which were correlated with the upregulation of the DELLA genes RGA, GAI, RGL1, and RGL2. Genes of the ethylene signaling and abscission-associated pathways, such as EDF1/2/3/4, BOP1/2, and IDA, were repressed in 35S::CaFYF1/2 and 35S::CaFYF1/2+SRDX transgenic Arabidopsis plants. The delayed senescence and abscission of the flower organs in 35S::CaFYF1/2 and 35S::CaFYF1/2+SRDX transgenic Arabidopsis plants were unaffected by ethylene treatment. These results indicated that similar to Arabidopsis FYF, CaFYF1/2 also act as repressors in controlling floral organ senescence and abscission in transgenic Arabidopsis plants. In contrast, when 35S::CaFYF1/2 was converted to a potent activator by fusion with the VP16-AD motif, flower senescence and abscission were promoted in these 35S::CaFYF1/2+VP16 transgenic dominant-negative mutant Arabidopsis plants. Furthermore, once CaFYF1/2 was fused with the strong repressor domain SRDX, severe delayed flower senescence and abscission were observed in 35S::CaFYF1/2+SRDX transgenic Arabidopsis plants. Delayed flower senescence and abscission were observed in 35S::CaFYF1/2 transgenic Arabidopsis plants. To investigate the function of these two genes, we performed ectopic expression of CaFYF1/2 in Arabidopsis. 710-724 ISSN: 0735-9640 Subject: Arabidopsis, Cattleya, abscission, ethylene, flowering, molecular biology, mutants Abstract: Two orthologues of Arabidopsis FOREVER YOUNG FLOWER (FYF), CaFYF1 and CaFYF2, were identified from Cattleya intermedia. Ectopic Expression of Two FOREVER YOUNG FLOWER Orthologues from Cattleya Orchid Suppresses Ethylene Signaling and DELLA Results in Delayed Flower Senescence/Abscission and Reduced Flower Organ Elongation in Arabidopsis Author: Wei-Han Chen, Yung-I Lee, Chang-Hsien Yang Source: Plant molecular biology reporter 2018 v.36 no.5-6 pp.
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